Formulation And Testing Of Antibacterial Activity Of Rambutan Leaves (Nephelium Lappaceum Linn) Ethanol Extract Ointment Against The Bacteria Staphylococcus Aureus

Abstract

Rambutan leaf samples were used in this research because people only think that rambutan leaves are just trash that is often thrown away and burned. This research aims to formulate the ethanol extract of rambutan leaves (Nephelium lappaceum Linn) into an ointment preparation, as well as testing the antibacterial activity against Staphylococcus aureus bacteria by using the paper disk method. In this research, 4 ointment formulations were made with different concentrations, namely F0 (0%), F1 (10%), F2 (20%), F3 (30%). Then a physical stability test was carried out on the ointment preparation for 4 weeks with test parameters including organoleptic test, pH test, homogeneity test, adhesion test, spreadability test, viscosity test, irritation test and hedonic test for each of the 4 formulas made which showed the results. meet the requirements for the stability of ointment preparations. Furthermore, testing the antibacterial activity of ointment preparations with concentrations of 10%, 20%, 30% and K-, K+ resulted in inhibition zone diameter values ​​of 10.66 mm, 13.33 mm, 22 mm and 0 mm, 11 mm. It can be concluded that the ethanol extract ointment preparation of rambutan leaves can inhibit the growth of staphylococcus aureus bacteria. The maximum antibacterial activity effect of the ethanol extract of rambutan leaves (Nephelium lappaceum Linn) is at a concentration of 30%. With an inhibition zone of 22 mm.