Formulation Of Physical Stability Test And Antibacterial Activity Test Of Hand Sanitizer Spray Ethanol Extract Of Biddara Leaves (Ziziphus Mauritiana L.) Against Staphylococcus Aureus Bacteria

Abstract

Bidara leaves (Ziziphus mauritiana L.) are a plant that has antibacterial activity, one of which is against staphylococcus aureus bacteria, so it can be formulated as an active substance in spray hand sanitizer preparations. This research aims to formulate bidara leaf extract and test its antibacterial activity against staphylococcus aureus bacteria using the disc diffusion method. In this research, 4 formulas were made with varying concentrations of bidara leaf extract (Ziziphus mauritiana L.). Then a physical stability test was carried out with test parameters including organoleptic, pH, homogeneity, freezer and thaw, irritation. The results of research based on the physical stability test showed that organoleptically, pH, homogeneity, irritation, for the 4 formulas, they were stable during storage in the freezer and thaw tests at F0 and F1 remained stable at F2 and F3, physical changes occurred, namely slightly viscous. Furthermore, based on the antibacterial spray hand sanitizer test against staphylococcus aureus, the results showed that the concentration of bidara leaf extract had an effect on inhibiting the growth of staphylococcus aureus bacteria with an inhibitory power of 14.9 mm for a concentration of 0.7%, 17.3 mm for a concentration of 1.4% and 19.75 mm for a concentration of 2.1%.